Publications
Highlighted works
Selected publications from the Lyssiotis lab for those looking to get a sense of our major research topics in the lab. For the most up to date list of publications in the lab, Costas Lyssiotis’ pubmed profile can be found here.
Selected publications:
Uridine-derived ribose fuels glucose-restricted pancreatic cancer
Nwosu ZC, Ward MH, Sajjakulnukit P, Poudel P, Ragulan C, Kasperek S, Radyk M, Sutton D, Menjivar RE, Andren A, Apiz-Saab JJ, Tolstyka Z, Brown K, Lee HJ, Dzierozynski LN, He X, Ps H, Ugras J, Nyamundanda G, Zhang L, Halbrook CJ, Carpenter ES, Shi J, Shriver LP, Patti GJ, Muir A, Pasca di Magliano M, Sadanandam A, Lyssiotis CA. Nature (2023) Jun;618(7963):151-158.
Abstract | PDF
OXPHOS promotes apoptotic resistance and cellular persistence in TH17 cells in the periphery and tumor microenvironment
Hong HS, Mbah NE, Shan M, Loesel K, Lin L, Sajjakulnukit P, Correa LO, Andren A, Lin J, Hayashi A, Magnuson B, Chen J, Li Z, Xie Y, Zhang L, Goldstein DR, Carty SA, Lei YL, Opipari AW, Argüello RJ, Kryczek I, Kamada N, Zou W, Franchi L, Lyssiotis CA. Sci Immunol. (2022) Nov 25;7(77):eabm8182.
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Differential integrated stress response and asparagine production drive symbiosis and therapy resistance of pancreatic adenocarcinoma cells
Halbrook CJ, Thurston G, Boyer S, Anaraki C, Jiménez JA, McCarthy A, Steele NG, Kerk SA, Hong HS, Lin L, Law FV, Felton C, Scipioni L, Sajjakulnukit P, Andren A, Beutel AK, Singh R, Nelson BS, Van Den Bergh F, Krall AS, Mullen PJ, Zhang L, Batra S, Morton JP, Stanger BZ, Christofk HR, Digman MA, Beard DA, Viale A, Zhang J, Crawford HC, Pasca di Magliano M, Jorgensen C, Lyssiotis CA. Nat Cancer (2022) Nov;3(11):1386-1403.
Abstract | PDF
Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context
Kerk SA, Lin L, Myers AL, Sutton DJ, Andren A, Sajjakulnukit P, Zhang L, Zhang Y, Jiménez JA, Nelson BS, Chen B, Robinson A, Thurston G, Kemp SB, Steele NG, Hoffman MT, Wen HJ, Long D, Ackenhusen SE, Ramos J, Gao X, Nwosu ZC, Galban S, Halbrook CJ, Lombard DB, Piwnica-Worms DR, Ying H, Pasca di Magliano M, Crawford HC, Shah YM, Lyssiotis CA. Elife (2022) Jul 11;11:e73245.
Abstract | PDF
Hyaluronic acid fuels pancreatic cancer cell growth
Kim PK, Halbrook CJ, Kerk SA, Radyk M, Wisner S, Kremer DM, Sajjakulnukit P, Andren A, Hou SW, Trivedi A, Thurston G, Anand A, Yan L, Salamanca-Cardona L, Welling SD, Zhang L, Pratt MR, Keshari KR, Ying H, Lyssiotis CA. Elife (2021) Dec 24;10:e62645.
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Selected Reviews:
Pancreatic cancer: Advances and challenges
Halbrook CJ, Lyssiotis CA, Pasca di Magliano M, Maitra A. Cell (2023) 186(8):1729-1754.
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Metabolic regulation of ferroptosis in the tumor microenvironment
Mbah NE, Lyssiotis CA. J Biol Chem. (2022) Mar;298(3):101617.
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Metabolic networks in mutant KRAS-driven tumours: tissue specificities and the microenvironment
Kerk SA, Papagiannakopoulos T, Shah YM, Lyssiotis CA. Nat Rev Cancer (2021) Aug;21(8):510-525.
Abstract | PDF
A guide to interrogating immunometabolism
Voss K, Hong HS, Bader JE, Sugiura A, Lyssiotis CA, Rathmell JC. Nat Rev Immunol. (2021) Oct;21(10):637-652.
Abstract | PDF
Metabolic Interactions in the Tumor Microenvironment
Lyssiotis CA, Kimmelman AC. Trends Cell Biol. (2017) Nov;27(11):863-875.
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Selected Preprints:
A Metabolic Atlas of Mouse Aging
Pilley SE, Awad D, Latumalea D, Esparza E, Zhang L, Shi X, Unfried M, Wang S, Mulondo R, Kashyap SB, Moaddeli D, Sajjakulnukit P, Sutton D, Wong H, Zhang Z, Coakley A, Garcia G, Higuchi-Sanabria R, Liu S, Yu B, Tu WB, Kennedy B, †Lyssiotis CA [†co-senior authors], †Mullen PJ. bioRxiv
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Glucose-6-phosphate dehydrogenase deficiency accelerates pancreatic acinar-to-ductal metaplasia
Radyk MD, Nelson BS, Halbrook CJ, Wood A, Lavoie B, Salvatore L, Corfas G, Colacino JA, Shah YM, Crawford HC, Lyssiotis CA. bioRxiv
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Targeting PIKfyve-driven lipid homeostasis as a metabolic vulnerability in pancreatic cancer
Cheng C, Hu J, Mannan R, Bhattacharyya R, Rossiter NJ, Magnuson B, Wisniewski JP, Zheng Y, Xiao L, Li C, Awad D, Korkaya A, He T, Bao Y, Zhang Y, Cao X, Wang Z, Mehra R, Morlacchi P, Sahai V, Pasca di Magliano M, Ding K, †Qiao Y, †Lyssiotis CA [†co-senior authors]. bioRxiv
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BRD4-mediated epigenetic regulation of endoplasmic reticulum-mitochondria contact sites is governed by the Coenzyme Q redox state
Chen B, Nguyen TM, Jadhav P, Halligan BS, Rossiter NJ, Guerra RM, Koo I, Hanna DA, Lin J, Banerjee R, Pagliarini DJ, Patterson AD, Mosalaganti S, Sexton JZ, Calì T, †Lyssiotis CA [†co-senior authors], †Shah YM. bioRxiv
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Multi-dimensional analyses identify genes of high priority for pancreatic cancer research
Nwosu ZC, Giza H, Nassif M, Charlestin V, Menjivar RE, Kim D, Kemp SB, Steele NG, Hu J, Hu B, Wang S, †Pasca di Magliano M, †Lyssiotis CA. bioRxiv
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All Publications
2024
A Metabolic Atlas of Mouse Aging
Pilley SE, Awad D, Latumalea D, Esparza E, Zhang L, Shi X, Unfried M, Wang S, Mulondo R, Kashyap SB, Moaddeli D, Sajjakulnukit P, Sutton D, Wong H, Zhang Z, Coakley A, Garcia G, Higuchi-Sanabria R, Liu S, Yu B, Tu WB, Kennedy B, †Lyssiotis CA [†co-senior authors], †Mullen PJ. bioRxiv
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Targeting PIKfyve-driven lipid homeostasis as a metabolic vulnerability in pancreatic cancer
Cheng C, Hu J, Mannan R, Bhattacharyya R, Rossiter NJ, Magnuson B, Wisniewski JP, Zheng Y, Xiao L, Li C, Awad D, Korkaya A, He T, Bao Y, Zhang Y, Cao X, Wang Z, Mehra R, Morlacchi P, Sahai V, Pasca di Magliano M, Ding K, †Qiao Y, †Lyssiotis CA [†co-senior authors]. bioRxiv
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BRD4-mediated epigenetic regulation of endoplasmic reticulum-mitochondria contact sites is governed by the Coenzyme Q redox state
Chen B, Nguyen TM, Jadhav P, Halligan BS, Rossiter NJ, Guerra RM, Koo I, Hanna DA, Lin J, Banerjee R, Pagliarini DJ, Patterson AD, Mosalaganti S, Sexton JZ, Calì T, †Lyssiotis CA [†co-senior authors], †Shah YM. bioRxiv
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2023
Glucose-6-phosphate dehydrogenase deficiency accelerates pancreatic acinar-to-ductal metaplasia
Radyk MD, Nelson BS, Halbrook CJ, Wood A, Lavoie B, Salvatore L, Corfas G, Colacino JA, Shah YM, Crawford HC, Lyssiotis CA. bioRxiv
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Pancreatic cancer: Advances and challenges
Halbrook CJ, Lyssiotis CA, Pasca di Magliano M, Maitra A. Cell (2023) 186(8):1729-1754.
Abstract | PDF
Spotlight on GOT2 in Cancer Metabolism
Kerk SA, Garcia-Bermudez J, Birsoy K, Sherman MH, Shah YM, Lyssiotis CA. Onco Targets Ther. (2023) Aug 22;16:695-702.
doi: 10.2147/OTT.S382161. PMID: 37635751; PMCID: PMC10460182.
Uridine-derived ribose fuels glucose-restricted pancreatic cancer
Nwosu ZC, Ward MH, Sajjakulnukit P, Poudel P, Ragulan C, Kasperek S, Radyk M, Sutton D, Menjivar RE, Andren A, Apiz-Saab JJ, Tolstyka Z, Brown K, Lee HJ, Dzierozynski LN, He X, Ps H, Ugras J, Nyamundanda G, Zhang L, Halbrook CJ, Carpenter ES, Shi J, Shriver LP, Patti GJ, Muir A, Pasca di Magliano M, Sadanandam A, Lyssiotis CA. Nature (2023) Jun;618(7963):151-158.
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Metabolomic Profiles of Human Glioma Inform Patient Survival
Scott AJ, Correa LO, Edwards DM, Sun Y, Ravikumar V, Andren AC, Zhang L, Srinivasan S, Jairath N, Verbal K, Muraszko K, Sagher O, Carty SA, Hervey-Jumper S, Orringer D, Kim MM, Junck L, Umemura Y, Leung D, Venneti S, Camelo-Piragua S, Lawrence TS, Ippolito JE, Al-Holou WN, Chinnaiyan P, Heth J, Rao A, Lyssiotis CA, Wahl DR. Antioxid Redox Signal. (2023) Nov;39(13-15):942-956.
doi: 10.1089/ars.2022.0085. Epub 2023 Apr 27. Erratum in: Antioxid Redox Signal. 2024 Apr 18;: PMID: 36852494; PMCID: PMC10655010.
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PTEN-induced kinase PINK1 supports colorectal cancer growth by regulating the labile iron pool
Chen B, Das NK, Talukder I, Singhal R, Castillo C, Andren A, Mancias JD, Lyssiotis CA, Shah YM. J Biol Chem. (2023) May;299(5):104691.
doi: 10.1016/j.jbc.2023.104691. Epub 2023 Apr 8. PMID: 37037306; PMCID: PMC10196865.
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Nutrient transporters: connecting cancer metabolism to therapeutic opportunities
Nwosu ZC, Song MG, di Magliano MP, Lyssiotis CA, Kim SE. Oncogene (2023) Mar;42(10):711-724.
doi: 10.1038/s41388-023-02593-x. Epub 2023 Feb 4. PMID: 36739364; PMCID: PMC10266237.
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2022
OXPHOS promotes apoptotic resistance and cellular persistence in TH17 cells in the periphery and tumor microenvironment
Hong HS, Mbah NE, Shan M, Loesel K, Lin L, Sajjakulnukit P, Correa LO, Andren A, Lin J, Hayashi A, Magnuson B, Chen J, Li Z, Xie Y, Zhang L, Goldstein DR, Carty SA, Lei YL, Opipari AW, Argüello RJ, Kryczek I, Kamada N, Zou W, Franchi L, Lyssiotis CA. Sci Immunol. (2022) Nov 25;7(77):eabm8182.
Abstract | PDF
Differential integrated stress response and asparagine production drive symbiosis and therapy resistance of pancreatic adenocarcinoma cells
Halbrook CJ, Thurston G, Boyer S, Anaraki C, Jiménez JA, McCarthy A, Steele NG, Kerk SA, Hong HS, Lin L, Law FV, Felton C, Scipioni L, Sajjakulnukit P, Andren A, Beutel AK, Singh R, Nelson BS, Van Den Bergh F, Krall AS, Mullen PJ, Zhang L, Batra S, Morton JP, Stanger BZ, Christofk HR, Digman MA, Beard DA, Viale A, Zhang J, Crawford HC, Pasca di Magliano M, Jorgensen C, Lyssiotis CA. Nat Cancer (2022) Nov;3(11):1386-1403.
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Amino acid metabolism in primary bone sarcomas
Jiménez JA, Lawlor ER, Lyssiotis CA. Front Oncol. (2022) Oct 5;12:1001318.
doi: 10.3389/fonc.2022.1001318. PMID: 36276057; PMCID: PMC9581121.
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Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context
Kerk SA, Lin L, Myers AL, Sutton DJ, Andren A, Sajjakulnukit P, Zhang L, Zhang Y, Jiménez JA, Nelson BS, Chen B, Robinson A, Thurston G, Kemp SB, Steele NG, Hoffman MT, Wen HJ, Long D, Ackenhusen SE, Ramos J, Gao X, Nwosu ZC, Galban S, Halbrook CJ, Lombard DB, Piwnica-Worms DR, Ying H, Pasca di Magliano M, Crawford HC, Shah YM, Lyssiotis CA. Elife (2022) Jul 11;11:e73245.
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Targeting allosteric regulation of cancer metabolism
Kremer DM, Lyssiotis CA. Nat Chem Biol. (2022) May;18(5):441-450. doi: 10.1038/s41589-022-00997-6. Epub 2022 Apr 28. PMID: 35484254.
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Discovery and Characterization of a Novel Allosteric Small-Molecule Inhibitor of NADP+-Dependent Malic Enzyme 1
Yoshida T, Kawabe T, Cantley LC, Lyssiotis CA. Biochemistry (2022) Aug 2;61(15):1548-1553.
doi: 10.1021/acs.biochem.2c00123. Epub 2022 Jul 12. PMID: 35819845; PMCID: PMC9352307.
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Multiomic characterization of pancreatic cancer-associated macrophage polarization reveals deregulated metabolic programs driven by the GM-CSF-PI3K pathway. Boyer S, Lee HJ, Steele N, Zhang L, Sajjakulnukit P, Andren A, Ward MH, Singh R, Basrur V, Zhang Y, Nesvizhskii AI, Pasca di Magliano M, Halbrook CJ, Lyssiotis CA. Elife. 2022 Feb 14;11:e73796. doi: 10.7554/eLife.73796. PMID: 35156921
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In an era of ctDNA, is metabolomics the new kid on the block?
Van Tine BA, Lyssiotis CA. Cancer Res. 2022 Feb 9:clincanres.4180.2022. doi: 10.1158/1078-0432.CCR-21-4180. Epub ahead of print. PMID: 35140120.
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Metabolic regulation of ferroptosis in the tumor microenvironment
Mbah NE, Lyssiotis CA. J Biol Chem. 2022 Jan 20:101617. doi: 10.1016/j.jbc.2022.101617. Epub ahead of print. PMID: 35065965.
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Reuterin in the healthy gut microbiome suppresses colorectal cancer growth through altering redox balance
Bell HN, Rebernick RJ, Goyert J, Singhal R, Kuljanin M, Kerk SA, Huang W, Das NK, Andren A, Solanki S, Miller SL, Todd PK, Fearon ER, Lyssiotis CA, Gygi SP, Mancias JD, Shah YM. Cancer Cell. 2022 Feb 14;40(2):185-200.e6. doi: 10.1016/j.ccell.2021.12.001. Epub 2021 Dec 23. PMID: 34951957; PMCID: PMC8847337.
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A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H2S oxidation
Kumar R, Landry AP, Guha A, Vitvitsky V, Lee HJ, Seike K, Reddy P, Lyssiotis CA, Banerjee R. J Biol Chem. 2022 Jan;298(1):101435. doi: 10.1016/j.jbc.2021.101435. Epub 2021 Nov 19. PMID: 34808207
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2021
Multi-dimensional analyses identify genes of high priority for pancreatic cancer research
Nwosu ZC, Giza H, Nassif M, Charlestin V, Menjivar RE, Kim D, Kemp SB, Steele NG, Hu J, Hu B, Wang S, †Pasca di Magliano M, †Lyssiotis CA. bioRxiv
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A guide to interrogating immunometabolism
Voss K, Hong HS, Bader JE, Sugiura A, Lyssiotis CA, Rathmell JC. Nat Rev Immunol. (2021) Oct;21(10):637-652.
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The biological underpinnings of therapeutic resistance in pancreatic cancer
Beatty GL, Werba G, Lyssiotis CA, Simeone DM. Genes Dev. 2021 Jul 1;35(13-14):940-962. doi: 10.1101/gad.348523.121. Epub 2021 Jun 11. PMID: 34117095; PMCID: PMC8247606.
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Colorectal cancer cells utilize autophagy to maintain mitochondrial metabolism for cell proliferation under nutrient stress
Devenport SN, Singhal R, Radyk MD, Taranto JG, Kerk SA, Chen B, Goyert JW, Jain C, Das NK, Oravecz-Wilson K, Zhang L, Greenson JK, Chen YE, Soleimanpour SA, Reddy P, Lyssiotis CA, Shah YM. JCI Insight. 2021 Jun 17;6(14):e138835. doi: 10.1172/jci.insight.138835. PMID: 34138755; PMCID: PMC8328084.
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Hepcidin sequesters iron to sustain nucleotide metabolism and mitochondrial function in colorectal cancer epithelial cells
Schwartz AJ, Goyert JW, Solanki S, Kerk SA, Chen B, Castillo C, Hsu PP, Do BT, Singhal R, Dame MK, Lee HJ, Spence JR, Lakhal-Littleton S, Vander Heiden MG, Lyssiotis CA, Xue X, Shah YM. Nat Metab. 2021 Jul;3(7):969-982. doi: 10.1038/s42255-021-00406-7. Epub 2021 Jun 21. PMID: 34155415; PMCID: PMC8316354.
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Fatty acid synthesis is required for breast cancer brain metastasis
Ferraro GB, Ali A, Luengo A, Kodack DP, Deik A, Abbott KL, Bezwada D, Blanc L, Prideaux B, Jin X, Possada JM, Chen J, Chin CR, Amoozgar Z, Ferreira R, Chen I, Naxerova K, Ng C, Westermark AM, Duquette M, Roberge S, Lindeman NI, Lyssiotis CA, Nielsen J, Housman DE, Duda DG, Brachtel E, Golub TR, Cantley LC, Asara JM, Davidson SM, Fukumura D, Dartois VA, Clish CB, Jain RK, Vander Heiden MG. Nat Cancer. 2021 Apr;2(4):414-428. doi: 10.1038/s43018-021-00183-y. Epub 2021 Apr 1. Erratum in: Nat Cancer. 2021 Nov;2(11):1243. PMID: 34179825; PMCID: PMC8223728.
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Metabolic networks in mutant KRAS-driven tumours: tissue specificities and the microenvironment
Kerk SA, Papagiannakopoulos T, Shah YM, Lyssiotis CA. Nat Rev Cancer. 2021 Aug;21(8):510-525. doi: 10.1038/s41568-021-00375-9. Epub 2021 Jul 9. PMID: 34244683.
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GOT1 inhibition promotes pancreatic cancer cell death by ferroptosis
Kremer DM, Nelson BS, Lin L, Yarosz EL, Halbrook CJ, Kerk SA, Sajjakulnukit P, Myers A, Thurston G, Hou SW, Carpenter ES, Andren AC, Nwosu ZC, Cusmano N, Wisner S, Mbah NE, Shan M, Das NK, Magnuson B, Little AC, Savani MR, Ramos J, Gao T, Sastra SA, Palermo CF, Badgley MA, Zhang L, Asara JM, McBrayer SK, di Magliano MP, Crawford HC, Shah YM, Olive KP, Lyssiotis CA. Nat Commun. 2021 Aug 11;12(1):4860. doi: 10.1038/s41467-021-24859-2. PMID: 34381026; PMCID: PMC8357841.
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The human type 2 diabetes-specific visceral adipose tissue proteome and transcriptome in obesity
Carruthers NJ, Strieder-Barboza C, Caruso JA, Flesher CG, Baker NA, Kerk SA, Ky A, Ehlers AP, Varban OA, Lyssiotis CA, Lumeng CN, Stemmer PM, O’Rourke RW. Sci Rep. 2021 Aug 30;11(1):17394. doi: 10.1038/s41598-021-96995-0. PMID: 34462518; PMCID: PMC8405693.
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Clinical Targeting of Altered Metabolism in High-Grade Glioma
Scott AJ, Lyssiotis CA, Wahl DR. Cancer J. 2021 Sep-Oct 01;27(5):386-394. doi: 10.1097/PPO.0000000000000550. PMID: 34570453; PMCID: PMC8480512.
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Cyst fluid metabolites distinguish malignant from benign pancreatic cysts
Shi J, Yi Z, Jin L, Zhao L, Raskind A, Yeomans L, Nwosu ZC, Simeone DM, Lyssiotis CA, Stringer KA, Kwon RS. Neoplasia. 2021 Nov;23(11):1078-1088. doi: 10.1016/j.neo.2021.09.004. Epub 2021 Sep 25. PMID: 34583246; PMCID: PMC8479274.
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Mitochondrial complex II in intestinal epithelial cells regulates T cell-mediated immunopathology
Fujiwara H, Seike K, Brooks MD, Mathew AV, Kovalenko I, Pal A, Lee HJ, Peltier D, Kim S, Liu C, Oravecz-Wilson K, Li L, Sun Y, Byun J, Maeda Y, Wicha MS, Saunders TL, Rehemtulla A, Lyssiotis CA, Pennathur S, Reddy P. Nat Immunol. 2021 Nov;22(11):1440-1451. doi: 10.1038/s41590-021-01048-3. Epub 2021 Oct 22. PMID: 34686860.
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ATDC binds to KEAP1 to drive NRF2-mediated tumorigenesis and chemoresistance in pancreatic cancer
Purohit V, Wang L, Yang H, Li J, Ney G.M,Gumkowski E.R, Vaidya A.J, Wang A, Bhardwaj A, Zhao E, Dolgalev I, Zamperone A, Abel EV, Pasca Di Magliano M, Crawford HC, Diolaiti D, Papagiannakopoulos TY, Lyssiotis CA, Simeone DM. Genes Dev. 2021 Jan 14. doi: 10.1101/gad.344184.120. Epub ahead of print.
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Hyaluronic acid fuels pancreatic cancer cell growth
Kim PK, Halbrook CJ, Kerk SA, Radyk M, Wisner S, Kremer DM, Sajjakulnukit P, Andren A, Hou SW, Trivedi A, Thurston G, Anand A, Yan L, Salamanca-Cardona L, Welling SD, Zhang L, Pratt MR, Keshari KR, Ying H, Lyssiotis CA. Elife. 2021 Dec 24;10:e62645. doi: 10.7554/eLife.62645. PMID: 34951587; PMCID: PMC8730721.
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Medium-Chain Acyl-CoA Dehydrogenase Protects Mitochondria from Lipid Peroxidation in Glioblastoma
Puca F, Yu F, Bartolacci C, Pettazzoni P, Carugo A, Huang-Hobbs E, Liu J, Zanca C, Carbone F, Del Poggetto E, Gumin J, Dasgupta P, Seth S, Srinivasan S, Lang FF, Sulman EP, Lorenzi PL, Tan L, Shan M, Tolstyka ZP, Kachman M, Zhang L, Gao S, Deem AK, Genovese G, Scaglioni PP, Lyssiotis CA, Viale A, Draetta GF. Cancer Discov. 2021 Nov;11(11):2904-2923. doi: 10.1158/2159-8290.CD-20-1437. Epub 2021 May 26. PMID: 34039636; PMCID: PMC8711129.
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Apolipoprotein E Promotes Immune Suppression in Pancreatic Cancer through NF-κB-Mediated Production of CXCL1
Kemp SB, Carpenter ES, Steele NG, Donahue KL, Nwosu ZC, Pacheco A, Velez-Delgado A, Menjivar RE, Lima F, The S, Espinoza CE, Brown K, Long D, Lyssiotis CA, Rao A, Zhang Y, Pasca di Magliano M, Crawford HC. Cancer Res. 2021 Aug 15;81(16):4305-4318. doi: 10.1158/0008-5472.CAN-20-3929. Epub 2021 May 28. PMID: 34049975; PMCID: PMC8445065.
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EWS-FLI1 and Menin Converge to Regulate ATF4 Activity in Ewing Sarcoma
Jiménez JA, Apfelbaum AA, Hawkins AG, Svoboda LK, Kumar A, Ruiz RO, Garcia AX, Haarer E, Nwosu ZC, Bradin J, Purohit T, Chen D, Cierpicki T, Grembecka J, Lyssiotis CA, Lawlor ER. Mol Cancer Res. (2021) Jul;19(7):1182-1195.
doi: 10.1158/1541-7786.MCR-20-0679. Epub 2021 Mar 19. PMID: 33741715; PMCID: PMC8462528.
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2020
Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer. Steele NG, Carpenter ES, Kemp SB, Sirihorachai V, The S, Delrosario L, Lazarus J, Amir ED, Gunchick V, Espinoza C, Bell S, Harris L, Lima F, Irizarry-Negron V, Paglia D, Macchia J, Chu AKY, Schofield H, Wamsteker EJ, Kwon R, Schulman A, Prabhu A, Law R, Sondhi A, Yu J, Patel A, Donahue K, Nathan H, Cho C, Anderson MA, Sahai V, Lyssiotis CA, Zou W, Allen BL, Rao A, Crawford HC, Bednar F, Frankel TL, Pasca di Magliano M. Nat Cancer. 2020 Nov;1(11):1097-1112. doi: 10.1038/s43018-020-00121-4. Epub 2020 Oct 26. PMID: 34296197; PMCID: PMC8294470.
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Cancer SLC43A2 alters T cell methionine metabolism and histone methylation.
Bian Y, Li W, Kremer DM, Sajjakulnukit P, Li S, Crespo J, Nwosu ZC, Zhang L, Czerwonka A, Pawłowska A, Xia H, Li J, Liao P, Yu J, Vatan L, Szeliga W, Wei S, Grove S, Liu JR, McLean K, Cieslik M, Chinnaiyan AM, Zgodziński W, Wallner G, Wertel I, Okła K, Kryczek I, Lyssiotis CA, Zou W. Nature. 2020 Sep;585(7824):277-282. doi: 10.1038/s41586-020-2682-1.
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Integrated Metabolic and Epigenomic Reprograming by H3K27M Mutations in Diffuse Intrinsic Pontine Gliomas.
Chung C, Sweha SR, Pratt D, Tamrazi B, Panwalkar P, Banda A, Bayliss J, Hawes D, Yang F, Lee HJ, Shan M, Cieslik M, Qin T, Werner CK, Wahl DR, Lyssiotis CA, Bian Z, Shotwell JB, Yadav VN, Koschmann C, Chinnaiyan AM, Blüml S, Judkins AR, Venneti S. Nature. 2020 Sep;585(7824):277-282. doi: 10.1038/s41586-020-2682-1.
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Purine metabolism regulates DNA repair and therapy resistance in glioblastoma.
Zhou W, Yao Y, Scott AJ, Wilder-Romans K, Dresser JJ, Werner CK, Sun H, Pratt D, Sajjakulnukit P, Zhao SG, Davis M, Nelson BS, Halbrook CJ, Zhang L, Gatto F, Umemura Y, Walker AK, Kachman M, Sarkaria JN, Xiong J, Morgan MA, Rehemtualla A, Castro MG, Lowenstein P, Chandrasekaran S, Lawrence TS, Lyssiotis CA, Wahl DR. Nat Commun. 2020 Jul 30;11(1):3811. doi: 10.1038/s41467-020-17512-x.
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Histone Acetyltransferase MOF Regulates Fatty Acid Oxidation and Cellular Metabolism in Cell Fate Determination.
Khoa LTP, Tsan Y-C, Mao F, Kremer MD, Sajjakulnukit P, Tong X, Bhanu NV, Garcia BA, Yin L, Smith GD, Saunders TL, Bielas S, Lyssiotis CA, Dou Y. Cell Stem Cell, 2020;20:30272-1.
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Common biochemical properties of metabolic genes recurrently dysregulated in tumors.
Oruganty K, Campit SE, Mamde S, Lyssiotis CA, Chandrasekaran S. Cancer & Metabolism, 2020;8:5.
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Integration of high-content fluorescence imaging into the metabolic flux assay reveals insights into mitochondrial properties and functions.
Little AC, Kovalenko I, Goo LE, Hong HS, Kerk S, Yates JA, Purohit V, Lombard D, Merajver S, Lyssiotis CA. Communications Biology, 2020;3:271.
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Endoplasmic reticulum-associated degradation regulates mitochondrial dynamics in brown adipocytes.
Zhou Z, Torres M, Sha H, Halbrook CJ, Van den Bergh F, Reinert RB, Yamada T, Wang S, Luo Y, Hunter AH, Wang C, Sanderson TH, Liu M, Taylor A, Sesaki H, Lyssiotis CA, Wu J, Kersten S, Beard DA, Qi L. Science, 2020;368:54-60.
Abstract | PDF
Severe metabolic alterations in liver cancer lead to ERK pathway activation and drug resistance.
Nwosu Z, Piorońska W, Battello N, Zimmer A, Dewidar B, Han M, Pereira S, Blagojevic B, Holenya P, Lochead J, De La Torre C, Gretz N, Sajjakulnukit P, Zhang L, Marquardt J, Pasca Di Magliano M, Lyssiotis CA, Ebert M, Sleeman J, Wölfl S, Meyer C, Hofmann U, Dooley S. EBiomedicine, 2020;102699.
Abstract | PDF
Small molecule activation of metabolic enzyme pyruvate kinase muscle isozyme 2, PKM2, provides photoreceptor neuroprotection.
Wubben TJ, Pawar M, Weh E, Smith A, Sajjakulnukit P, Zhang L, Dai L, Hager H, Pai MP, Lyssiotis CA, Besirli CG. Scientific Reports, 2020;10:2990.
Abstract | PDF
Cysteine depletion induces pancreatic tumor ferroptosis in mice.
Badgley MA, Kremer DM, Maurer HC, DelGiorno KE, Lee H-J, Purohit V, Sagalovskiy I, Firl CEM, Sastra SA, Palermo CF, Kapillian J, Decker A, Ma A, Sajjakulnukit P, Zhang L, Tolstyka ZP, Cerf TH, Liu T, Gu W, Seeley ES, Stone E, Georgiou G, Luga A, Wahl GM, Stockwell BR, Lyssiotis CA, Olive KP. Science, 2020;368:85-89.
Abstract | PDF
Regulatory T cell depletion causes compensatory immune suppression and accelerated pancreatic carcinogenesis.
Zhang Y, Lazarus J, Steele N, Lee H-J, Yan W, Halbrook CJ, Menjivar R, Kemp SB, Sirihorocai V, Carpenter ES, Nevison AC, Vinta A, Crawford HC, Lyssiotis CA, Frankel TL, Bednar F, Pasca di Magliano M. Cancer Discovery, 2020;10:422-439.
Abstract | PDF
Tissue of Origin Dictates GOT1 Dependence and Confers Synthetic Lethality to Radiotherapy.
Nelson BS, Lin L, Kremer DM, Sousa C, Cotta-Ramusino C, Myers A, Ramos J, Gao T, Kovalenko I, Wilder-Romans K, Dresser J, Davis M, Nwosu Z, Mashadova O, Nicolay B, Tolstyka ZP, Halbrook CJ, Asara JM, Crawford HC, Cantley LC, Kimmelman AC, Wahl D, Lyssiotis CA. Cancer & Metabolism, 2020;8:1.
Abstract | PDF
A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner.
Glowacki RWP, Pudlo NA, Tuncil Y, Luis AS, Sajjakulnukit P, Terekhov AI, Lyssiotis CA, Hamaker BR, Martens EC. Cell Host & Microbe, 2020;27:79-92
Abstract | PDF
A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth.
Yoshida T, Yamasaki S, Kaneko O, Taoka A, Tomimoto Y, Namatame I, Yahata T, Kuromitsu S, Cantley LC, Lyssiotis CA. BBRC, 2020;522:633-638
Abstract | PDF
2019
Inhibiting the biosynthesis of UDP-GlcNAc lowers O-GlcNAcylated levels and sensitizes cancer to environmental stress.
Walter LA, Lin YH, Halbrook CJ, Chuh KN, He L, Pedowitz NJ, Batt AR, Brennan CK, Stiles BL, Lyssiotis CA, Pratt MR. Biochemistry, 2019 [ePub ahead of print]
Abstract | PDF
NAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome.
Fang EF, Hou Y, Lautrup S, Jensen MB, Yang B, SenGupta T, Caponio D, Khezri R, Demarest TG, Aman Y, Figueroa D, Morevati M, Lee H-J, Kato H, Kassahun H, Lee J-H, Filippeli D, Okur MN, Mangerich A, Croteau DL, Maezawa Y, Lyssiotis CA, Tao J, Yokote K, Rusten TE, Mattson MP, Jasper H, Nilsen H, Bohr VA. Nature Communications, 2019;10:5284.
Abstract | PDF
Meta-analysis of targeted metabolomics data from heterogeneous biological samples provides insights into metabolite dynamics.
Lee H-J, Kremer DM, Sajjakulnukit P, Zhang L, Lyssiotis CA. Metabolomics, 2019;15:103.
Abstract | PDF
Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells
Libiad M, Bostelaar T, Vitvitsky V, Bak D, Lee H-J, Leprevost FdV, Sakamoto N, Nesvizhskii A, Chen G, Fearon E, Lyssiotis CA, Martens E, Werapana E, Banerjee R. JBC, 2019;294:12077-12090.
Abstract | PDF
Auditory metabolomics, an approach to identify acute molecular effects of noise trauma.
Ji L, Lee HJ, Wan G, Wang G, Zhang L, Sajjakulnukit P, Schacht J, †Lyssiotis CA [†co-senior authors], †Corfas G. Scientific Reports, 2019;9:9273.
Abstract | PDF
Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.
Li M, Chiang YL, Lyssiotis CA, Teater M, Hong JY, Shen H, Wang L, Hu J, Jing H, Chen Z, Jian N, Duy C, Mistry SJ, Cerchietti L, Cross JR, Cantley LC, Green MR, Lin H, Melnick AM. Cancer Cell, 2019;35:916-931.
Abstract | PDF
Abnormal oxidative metabolism in a quiet genomic backgorund underlies clear cell papillary renal cell carcinoma.
Xu J, Reznik E, Lee H-J, Gundem G, Jonsson P, Sarungbam J, Bialik A, Vega FS, Creighton C, Hoekstra JG, Zhang L, Sajjakulnukit P, Kremer DM, Tolstyka ZP, Casuscelli J, Stirdivant S, Tang J, Schultz N, Jeng PS, Dong Y, Su W, Cheng E, Russo P, Coleman J, Papaemmanuil E, Chen YB, Reuter VE, Sander C, Kennedy S, Hsieh J, †Lyssiotis CA [†co-senior authors], †Tickoo SK, †Hakimi AA. eLife, 2019;8:e38986.
Abstract | Full Article
Enhanced oxidative phosphorylation by PLZF is essential for survival and function of NKT cells.
Kumar A, Pyaram K, Yarosz E, Hong H, Lyssiotis CA, Giri S, Chang C-H.PNAS, 2019;116:7439-7448.
Abstract | PDF
Macrophage Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.
Halbrook CJ, Pontious C, Lee H-J, Kovalenko I, Zhang Y, Lapienyte L, Dreyer S, Kremer DL, Zhang L, Sajjakulnukit P, Zhang L, Nelson B, Hong H, Kemp S, Chang D, Biankin A, Crawford HC, Morton JP, Pasca di Magliano M, Lyssiotis CA. Cell Metabolism, 2019;29:1390-1399.
Abstract | PDF
ERK inhibition increases pancreatic cancer dependence on autophagy.
Bryant KL, Stalnecker CA, Zeitouni D, Klomp JE, Peng S, Tikunov AP, Gunda V, Pierobon M, Waters AM, George SD, Tomar G, Papke B, Hobbs GA, Yan L, Hayges TK, Diehl JN, Goode G, Chaika NV, Wang Y, Zhang G-F, Witkiewicz AK, Knudsen ES, Petricoin E, Singh PK, Macdonald JM, Tran NL, Lyssiotis CA, Ying H, Kimmelman AC, Cox AD, Der CJ Nature Medicine, 2019;25:628-640.
Abstract | Full Article
IDH1R132H acts as a tumor suppressor in glioma via epigenetic upregulation of the DNA damage response.
Núñez FJ, Mendez FM, Kadiyala P, Savelieff MG, Koschmann C, Calinescu A-A, Kamran N, Patel R, Carney S, Alghamri M, Edwards M, Ljungman M, Qin T, Sartor MA, Tagett R, Venneti S, Brosnan-Cashman J, Meeker A, Gorbunova V, Zhao L, Kremer DM, Zhang L, Lyssiotis CA, Hervey-Jumper S, Figueroa ME, Lowenstein PR, Castro MG. Science Translational Medicine, 2019;11:479.
Abstract | PDF
Protocol for 13C/15N Targeted Flux Analysis by LC-MS/MS for Ex Vivo and In Vivo Labeling of Central Carbon Metabolism and Related Pathways.
Yuan M, Kremer DM, Huang H, Ben Sahra I, Manning BD, †Lyssiotis CA [†co-senior authors], †Asara JM. Nature Protocols, 2019;14:313-330.
Abstract | PDF
2018
Oncogenic Kras supports pancreatic cancer through regulation of nucleotide synthesis.
Santana-Codinab N, Roeth AA, Zhang Y, Yang A, Asara JM, Wang X, Bronson RT, Lyssiotis CA, Ying H, Kimmelman AC. Nature Communications, 2018;9:4945.
Abstract | PDF
Tumor Crosstalk Networks Promote Growth and Support Immune Evasion in Pancreatic Cancer.
Halbrook CJ, Pasca di Magliano M, Lyssiotis CA. Am J Physiol Gastrointest Liver Physiol (2018) 315:G27-G35.
Abstract | PDF
Biochemical Characterization and Structure-based Mutational Analysis Provides Insight into Binding and Mechanism of Action of Novel Aspartate Aminotransferase Inhibitors.
Holt MC, Assar Z, Beheshti Zavareh R, Lin L, Anglin J, Mashadova O, Haldar D, Mullarky E, Kremer DM, Cantley LC, Kimmelman AC, Stein AJ, Lairson LL, Lyssiotis CA. Biochemistry (2018) EPub Ahead of Print.
Abstract | PDF
2017
Metabolic Interactions in the Tumor Microenvironment
Lyssiotis CA, Kimmelman AC. Trends in Cell Biology (2017) 27:863-875.
Abstract | PDF
Employing Metabolism to Improve the Diagnosis and Treatment of Pancreatic Cancer
Halbrook CJ, Lyssiotis CA. Cancer Cell (2017) 31:5-19.
Abstract | PDF
2016-2012
Pancreatic Stellate Cells Support Tumour Metabolism Through Autophagic Alanine Secretion.
Sousa CM, Biancur DE, Wang X, Halbrook CJ, Sherman MH, Zhang L, Kremer D, Hwang RF, Witkiewicz AK, Ying H, Asara JM, Evans RM, Cantley LC, *Lyssiotis CA [co-senior authors], *Kimmelman AC. Nature (2016) 25, 479-483.
Abstract | PDF
Targeting Glutamine Metabolism Sensitizes Pancreatic Cancer to PARP1-driven Metabolic Catastrophe Induced by ß-lapachone.
Chakrabarti G, Moore Z, Luo X, Ilcheva M, Ali A, Anderson GG, Padanad M, Scaglioni PP, Cantley LC, Kimmelman AC, DeBerardinis RJ, *Lyssiotis CA [co-senior authors], *Boothman DA. Cancer & Metabolism (2015) 3, 12.
Abstract | PDF
Oncogene Ablation-Resistant Pancreatic Cancer Cells Depend on Mitochondrial Function.
Viale A, Pettazzoni P, Lyssiotis CA, Ying H, Sanchez N, Marchesini M, Carugo A, Green T, Seth S, Giuliani V, Kost-Alimova M, Muller F, Colla S, Nezi L, Genovese G, Deem AK, Kapoor A, Carugo A, Yao W, Brunetto E, Kang Y, Yuan M, Asara JM, Wang YA, Heffernan TP, Kimmelman AC, Wang H, Fleming J, Cantley LC, DePinho R & Draetta G. Nature (2014) 30, 628–632.
Abstract | PDF | Extended Figures | Times cited: 252
Glutamine Supports Pancreatic Cancer Growth Through a KRAS-Regulated Metabolic Pathway.
*Son J, *Lyssiotis CA [*co-lead authors], Ying H, Wang X, Hua S, Ligorio M, Perera RM, Ferrone CR, Mullarky E, Shyh-Chang N, Kang Y, Fleming JB, Bardeesy N, Asara JM, Haigis MC, DePinho RA, Cantley LC & Kimmelman AC. Nature (2013) 496, 101–105.
Abstract | PDF | Supporting Information | Times cited: 506
Oncogenic Kras Maintains Pancreatic Tumors through Regulation of Anabolic Glucose Metabolism.
*Ying H, *Kimmelman AC, *Lyssiotis CA [*co-lead authors], Hua S, Chu GC, Fletcher-Sananikone E, Locasale JW, Son J, Zhang H, Coloff JL, Yan H, Wang W, Chen S, Viale A, Zheng H, Paik J, Lim C, Guimaraes AR, Martin ES, Chang J, Hezel AF, Perry SR, Hu J, Gan B, Xiao Y, Asara JM, Weissleder R, Wang YA, Chin L, Cantley LC & DePinho RA. Cell (2012) 149, 656–670.
Abstract | PDF | Supporting Information | Times cited: 592
Biochemical Characterization and Structure-based Mutational Analysis Provides Insight into Binding and Mechanism of Action of Novel Aspartate Aminotransferase Inhibitors
Holt MC, Assar Z, Beheshti Zavareh R, Lin L, Anglin J, Mashadova O, Haldar D, Mullarky E, Kremer DM, Cantley LC, Kimmelman AC, Stein AJ, Lairson LL, Lyssiotis CA. Biochemistry (2018) EPub Ahead of Print.
Abstract | PDF
Metabolic Interactions in the Tumor Microenvironment
Lyssiotis CA, Kimmelman AC. Trends in Cell Biology (2017) 27:863-875.
Abstract | PDF
Employing Metabolism to Improve the Diagnosis and Treatment of Pancreatic Cancer
Halbrook CJ, Lyssiotis CA. Cancer Cell (2017) 31:5-19.
Abstract | PDF
Pancreatic Stellate Cells Support Tumour Metabolism Through Autophagic Alanine Secretion
Sousa CM, Biancur DE, Wang X, Halbrook CJ, Sherman MH, Zhang L, Kremer D, Hwang RF, Witkiewicz AK, Ying H, Asara JM, Evans RM, Cantley LC, *Lyssiotis CA [co-senior authors], *Kimmelman AC. Nature (2016) 25, 479-483.
Abstract | PDF
Targeting Glutamine Metabolism Sensitizes Pancreatic Cancer to PARP1-driven Metabolic Catastrophe Induced by ß-lapachone
Chakrabarti G, Moore Z, Luo X, Ilcheva M, Ali A, Anderson GG, Padanad M, Scaglioni PP, Cantley LC, Kimmelman AC, DeBerardinis RJ, *Lyssiotis CA [co-senior authors], *Boothman DA. Cancer & Metabolism (2015) 3, 12.
Abstract | PDF
Oncogene Ablation-Resistant Pancreatic Cancer Cells Depend on Mitochondrial Function
Viale A, Pettazzoni P, Lyssiotis CA, Ying H, Sanchez N, Marchesini M, Carugo A, Green T, Seth S, Giuliani V, Kost-Alimova M, Muller F, Colla S, Nezi L, Genovese G, Deem AK, Kapoor A, Carugo A, Yao W, Brunetto E, Kang Y, Yuan M, Asara JM, Wang YA, Heffernan TP, Kimmelman AC, Wang H, Fleming J, Cantley LC, DePinho R & Draetta G. Nature (2014) 30, 628–632.
Abstract | PDF | Extended Figures | Times cited: 252
Glutamine Supports Pancreatic Cancer Growth Through a KRAS-Regulated Metabolic Pathway
*Son J, *Lyssiotis CA [*co-lead authors], Ying H, Wang X, Hua S, Ligorio M, Perera RM, Ferrone CR, Mullarky E, Shyh-Chang N, Kang Y, Fleming JB, Bardeesy N, Asara JM, Haigis MC, DePinho RA, Cantley LC & Kimmelman AC. Nature (2013) 496, 101–105.
Abstract | PDF | Supporting Information | Times cited: 506
Oncogenic Kras Maintains Pancreatic Tumors through Regulation of Anabolic Glucose Metabolism
*Ying H, *Kimmelman AC, *Lyssiotis CA [*co-lead authors], Hua S, Chu GC, Fletcher-Sananikone E, Locasale JW, Son J, Zhang H, Coloff JL, Yan H, Wang W, Chen S, Viale A, Zheng H, Paik J, Lim C, Guimaraes AR, Martin ES, Chang J, Hezel AF, Perry SR, Hu J, Gan B, Xiao Y, Asara JM, Weissleder R, Wang YA, Chin L, Cantley LC & DePinho RA. Cell (2012) 149, 656–670.
Abstract | PDF | Supporting Information | Times cited: 592